荧光团
蛋白酶
荧光
生物物理学
丙氨酸
肽
天冬酰胺
生物化学
化学
生物
酶
氨基酸
量子力学
物理
作者
Xi Li,Qingzhu Liu,Siqin Ye,Shijie Wang,Ké Li,Gaochao Lv,Ying Peng,Ling Qiu,Jianguo Lin
摘要
Abstract Legumain, a lysosomal cysteine protease, is critical for pathological progression and has been found to play an important role in the occurrence and development of several cancers. However, its biological functions remain few recognized. To further understand the role of legumain activity in tumor progression, a legumain protease‐responsive fluorescent probe was developed in the present study. The probe 1 was synthesized by conjugating an aminoluciferin fluorophore with an alanine–alanine–asparagine (AAN) peptide sequence. The successful synthesis of probe 1 was validated by NMR and MS spectra as well as HPLC analysis. The probe 1 was non‐toxic and exhibited great stability in the physiological solutions. More importantly, compared with the aminoluciferin fluorophore, the peptide conjugation may dramatically increase the targeting specificity. Probe 1 was able to effectively detect the legumain activity in living HCT116 cells through fluorescence imaging. All these results implied that probe 1 could act as a promising fluorescent probe specialized for the monitoring of legumain activity in living cells.
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