已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Identification and function analysis of bHLH genes in response to cold stress in sweetpotato

基因 生物 遗传学 基因家族 基因复制 非生物胁迫 转录因子 功能(生物学) 串联外显子复制 甘薯 基因表达 植物
作者
Rong Jin,Ho Soo Kim,Tao Yu,Aijun Zhang,Yukui Zhang,Ming Liu,Wenhui Yu,Peng Zhao,Qiangqiang Zhang,Qinghe Cao,Sang‐Soo Kwak,Zhonghou Tang
出处
期刊:Plant Physiology and Biochemistry [Elsevier]
卷期号:169: 224-235 被引量:22
标识
DOI:10.1016/j.plaphy.2021.11.027
摘要

Basic/helix-loop-helix (bHLH) transcription factors are involved in various metabolic and physiological processes in plants. Sweetpotato (Ipomoea batatas (L.) Lam.) is an important crop in China but is highly susceptible to cold stress. However, little information on the bHLH gene family is available, and the function of this family in response to cold stress has not been revealed in sweetpotato. Here, 110 IbbHLHs were identified and classified into 17 categories based on phylogenetic relationships, conserved motifs and gene structure analyses. Except for 5 IbbHLHs, 90 IbbHLHs were putative E-box-binding proteins including 70 IbbHLHs belonging to G-box, whereas 15 IbbHLHs were putative non-E box-binding proteins based on DNA-binding analysis. In total, 37 pairs of segmental duplicated genes and 5 pairs of tandem duplication genes were identified within the IbbHLH gene family. The transcript level of 20 IbbHLHs was regulated by cold stress based on RNA-seq data, and 8 genes were selected for further quantitative real-time PCR (qRT-PCR) analysis. IbHLH8 and IbHLH92 are involved in network interaction with several genes related to abiotic and biotic stresses under cold treatment. IbbHLH79, an ICE1-like gene, was isolated and overexpressed in sweetpotato. The IbbHLH79 protein can activate the CBF (C-repeat Binding Factor) pathway, and IbbHLH79-overexpressing transgenic plants display enhanced cold tolerance. Taken together, these results provide valuable information on the IbbHLH gene family; in addition, several IbbHLHs may regulate cold stress, and the results suggest IbbHLH79 will be useful for molecular breeding of enhanced cold tolerance in sweetpotato.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
传奇3应助弄香采纳,获得10
1秒前
冷酷哈密瓜完成签到,获得积分10
6秒前
儒雅的焦发布了新的文献求助10
7秒前
10秒前
坚强豪英完成签到,获得积分10
12秒前
13秒前
卡恩完成签到 ,获得积分10
14秒前
今天鱼怎么样完成签到 ,获得积分10
16秒前
现实的幻露完成签到 ,获得积分10
17秒前
bionova完成签到,获得积分10
20秒前
机灵鼠标发布了新的文献求助10
20秒前
FFF完成签到,获得积分10
22秒前
allshestar完成签到 ,获得积分10
22秒前
韩较瘦完成签到,获得积分10
24秒前
Singularity应助科研通管家采纳,获得10
25秒前
汉堡包应助科研通管家采纳,获得10
25秒前
25秒前
打打应助科研通管家采纳,获得10
25秒前
25秒前
不安青牛应助科研通管家采纳,获得10
25秒前
VDC应助科研通管家采纳,获得10
26秒前
Jasper应助科研通管家采纳,获得10
26秒前
SciGPT应助科研通管家采纳,获得10
26秒前
不安青牛应助科研通管家采纳,获得10
26秒前
Lucas应助科研通管家采纳,获得30
26秒前
深情安青应助科研通管家采纳,获得10
26秒前
Owen应助科研通管家采纳,获得10
26秒前
不安青牛应助科研通管家采纳,获得10
26秒前
华仔应助科研通管家采纳,获得10
26秒前
星辰大海应助科研通管家采纳,获得10
26秒前
安安应助科研通管家采纳,获得10
26秒前
机灵鼠标完成签到,获得积分20
29秒前
TTTaT完成签到,获得积分10
30秒前
bionova发布了新的文献求助30
36秒前
彦卿完成签到 ,获得积分10
40秒前
研友_Z72Ydn完成签到 ,获得积分10
48秒前
ooo完成签到,获得积分20
49秒前
从容芮应助PGD采纳,获得100
50秒前
50秒前
50秒前
高分求助中
Evolution 10000
ISSN 2159-8274 EISSN 2159-8290 1000
Becoming: An Introduction to Jung's Concept of Individuation 600
Ore genesis in the Zambian Copperbelt with particular reference to the northern sector of the Chambishi basin 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3161905
求助须知:如何正确求助?哪些是违规求助? 2813139
关于积分的说明 7898729
捐赠科研通 2472140
什么是DOI,文献DOI怎么找? 1316366
科研通“疑难数据库(出版商)”最低求助积分说明 631278
版权声明 602129