菌丝体
蘑菇
生物
酵母
食品科学
麸皮
酵母抽提物
伞菌纲
植物
擔子菌門
生物化学
发酵
原材料
生态学
作者
Tianxu Cao,Shanshan Luo,Ping Du,Hui Tu,Qian Zhang
出处
期刊:International Journal of Medicinal Mushrooms
日期:2023-01-01
卷期号:25 (10): 77-90
被引量:1
标识
DOI:10.1615/intjmedmushrooms.2023049499
摘要
Pholiota adiposa is an important edible and medicinal mushroom with high nutritional and medicinal effects. The fruiting body of wild fungi collected from Mudanjiang City, Heilongjiang Province, was identified by morphological description and molecular identification, the biological characteristics and domestication of the fungus was determined by single factor and orthogonal tests. The wild strain isolated was determined to be Ph. adiposa based on morphological characteristics, sequence alignment between ITS and nLSU, and phylogenetic relationship analysis. The single factor results revealed that the optimal carbon source, nitrogen source, culture temperature, and pH for the mycelia growth of Ph. adiposa were glucose, yeast paste, 25°C, and pH 6.5-7.0, respectively. Orthogonal test showed that the optimal formula for mycelia culture was fructose 20 g/L, yeast extract 6 g/L, KH2PO4 1 g/L and MgSO4 2.5 g/L. The highest single bag yield of the fruiting body of Ph. adiposa was 24.96 g in the culture medium formula of sawdust (20%), wheat bran (10%), soybean powder (1%), and quicklime powder (1%). The results will provide basic information for the protection, utilization and domestication of the resources of Ph. adiposa.
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