两性霉素B
化学
色谱法
蛋白质沉淀
药代动力学
脂质体
洗脱
检出限
分析物
药理学
抗真菌
皮肤病科
生物化学
医学
作者
Quanyu Xu,Yueyue Qian,Ye Yuan,Qing Shao,Xuejun He,Hongqun Qiao
出处
期刊:Current Pharmaceutical Analysis
[Bentham Science]
日期:2019-04-15
卷期号:16 (7): 880-891
标识
DOI:10.2174/1573412915666190415150644
摘要
Background:: Among the existing antifungal drugs, Amphotericin B is the first drug in the treatment of systemic fungal infections. However, its large adverse reactions limit the clinical application and Liposome Amphotericin B resolves the problem. Objective:: In the present study, a rapid, simple, sensitive and efficient method based on LCMS/ MS for determination of liposomal Amphotericin B in rat plasma and tissue samples using natamycin as the internal standard has been developed and validated. Methods: The analytical samples contain the plasma and various tissues disposed of by protein precipitation and determination of liposomal Amphotericin B by an LC-MS/MS. Chromatographic separation was achieved on a Poroshell 120 EC-C18 column (4.6 mm × 50 mm, 2.7 μm) with 10 mmol/L ammonium acetate in water-acetonitrile by gradient elution at a flow rate of 0.7 mL/min. The MS analysis was conducted in positive electrospray ionization with Multiple Reaction Monitoring (MRM). Results:: The calibration curves of plasma and tissues showed good linear range from 50 to 10000 ng/mL. The analytical samples containing plasma and tissues were stable under different storage conditions and temperature. Conclusions: : The developed LC-MS/MS method has been successfully applied to the studies of toxicokinetics and tissue distribution after intravenous injection of liposomal Amphotericin B to rats.
科研通智能强力驱动
Strongly Powered by AbleSci AI