类有机物
LGR5型
溃疡性结肠炎
生物
炎症性肠病
肠粘膜
信使核糖核酸
免疫学
病理
分子生物学
干细胞
疾病
细胞生物学
基因
医学
内科学
遗传学
癌症干细胞
作者
Manuel Noben,Bram Verstockt,Magali de Bruyn,Nikolai Hendriks,Gert Van Assche,Séverine Vermeire,Catherine M. Verfaillie,Marc Ferrante
出处
期刊:Gut
[BMJ]
日期:2017-02-03
卷期号:66 (12): 2193-2195
被引量:39
标识
DOI:10.1136/gutjnl-2016-313667
摘要
With great interest we read the paper by Dotti et al ,1 who identified a set of differentially expressed genes in epithelial organoid cultures (EpOCs) derived from patients with UC compared with EpOCs from healthy controls.
Similarly, we created an EpOC library from patients with UC (n=17), Crohn's disease (CD, n=12) and healthy controls (n=10). First, we assessed the potential of isolated intestinal crypts to grow into organoids (colony forming units). We observed that a similar percentage of organoids was formed from intestinal crypts isolated from controls and patients with UC or CD. Moreover, intestinal crypts isolated from macroscopically inflamed and non-inflamed tissue had similar potential to form organoids (figure 1A). Although we used a slightly divergent differentiation medium,2 the expansion and differentiation capacity of our organoids was similar as demonstrated by Dotti et al .
Figure 1
(A) Colony forming units (CFUs) determined by quantification of the number of seeded crypts at the day of isolation and organoid formation after 7 days. CFUs are expressed as a percentage of organoids representative to the original number of seeded crypts. (B) Relative mRNA expression levels of leucine-rich repeat-containing G-protein coupled receptor 5 ( LGR5 ), mucin2 ( MUC2 ) and protein atonal homologue 1 ( ATOH1 ). mRNA levels were …
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