Pulsed ultrasound assisted extraction of alternative plant protein from sugar maple leaves: Characterization of physical, structural, thermal, electrical, and techno-functional properties

Plant proteins are gaining in popularity and are increasingly being considered as an alternative to animal protein , thus a sustainable, yet comparable source of plant protein is highly recommended. To this end, the current study was conducted to investigate the structural, thermal, physical, and functional attributes, as well as the volatile profile of sugar maple leaves (SML) protein. To this end, SML protein was extracted by homogenization (10000 rpm, 5 min) pretreated ultrasound-assisted extraction (120000 J, 60% amplitude, 5:5 pulse, 25 °C, and 15 min) at varying pH (8−10) and compared with conventionally extracted SML protein. Among the different protein extracts, sonicated extract (pH9) provided a good protein yield (up to 7%) having higher protein content (210 mg Bovine serum albumin/g dry matter), thermal stability (onset of denaturation temperature:114 °C), and functional properties (solubility, emulsion capacity, water holding capacity , and antioxidant activity). On contrary, the characteristic green aroma, caused by 3-hexen-1-ol and nonanal , was found higher in sonicated protein extracts than that of macerated extracts. Having said that, this study showed the versatile properties of SML protein fractions that can be used as plant-based food ingredients. • Sugar maple leaf (SML) is a potential and sustainable source of plant-based protein. • SML protein was extracted by pulsed ultrasound-assisted extraction. • Homogenization pretreatment enhanced protein yield. • Protein denaturation temperature enhanced by ultrasonication. • Sonication improved the SML protein functional and antioxidant properties.