作者
Christopher M. Bartley,Thomas T. Ngo,Le-Duy Do,Αναστασία Ζεκερίδου,Ravi Dandekar,Sergio Muñiz‐Castrillo,Bonny D. Alvarenga,Kelsey C. Zorn,Asritha Tubati,Anne‐Laurie Pinto,Weston D. Browne,Patrick W. Hullett,Mark Terrelonge,Ryan Schubert,Amanda L. Piquet,Binxia Yang,Mayra Montalvo,Andrew F. Kung,Sabrina A Mann,Maulik Shah,Michael D. Geschwind,Jeffrey M. Gelfand,Joseph L. DeRisi,Sean J. Pittock,Jérôme Honnorat,Samuel J. Pleasure,Michael R. Wilson
摘要
Objective Co‐occurring anti‐tripartite motif‐containing protein 9 and 67 autoantibodies (TRIM9/67‐IgG) have been reported in only a very few cases of paraneoplastic cerebellar syndrome. The value of these biomarkers and the most sensitive methods of TRIM9/67‐IgG detection are not known. Methods We performed a retrospective, multicenter study to evaluate the cerebrospinal fluid and serum of candidate TRIM9/67‐IgG cases by tissue‐based immunofluorescence, peptide phage display immunoprecipitation sequencing, overexpression cell‐based assay (CBA), and immunoblot. Cases in which TRIM9/67‐IgG was detected by at least 2 assays were considered TRIM9/67‐IgG positive. Results Among these cases (n = 13), CBA was the most sensitive (100%) and revealed that all cases had TRIM9 and TRIM67 autoantibodies. Of TRIM9/67‐IgG cases with available clinical history, a subacute cerebellar syndrome was the most common presentation (n = 7/10), followed by encephalitis (n = 3/10). Of these 10 patients, 70% had comorbid cancer (7/10), 85% of whom (n = 6/7) had confirmed metastatic disease. All evaluable cancer biopsies expressed TRIM9 protein (n = 5/5), whose expression was elevated in the cancerous regions of the tissue in 4 of 5 cases. Interpretation TRIM9/67‐IgG is a rare but likely high‐risk paraneoplastic biomarker for which CBA appears to be the most sensitive diagnostic assay. ANN NEUROL 2023;94:1086–1101