已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Gleditsiae sinensis fructus Pills combined with Jujubae fructus attenuate chronic bronchitis via regulation of AGE-RAGE signaling pathway

药理学 慢性支气管炎 免疫印迹 医学 支气管肺泡灌洗 中医药 传统医学 信号转导 姜黄素 支气管炎 化学 病理 内科学 生物化学 基因 替代医学
作者
Changchang Wang,Le Kang,Bei-lei Zuo,Xin Peng,Hongwei Li,Ning Zhou,Kai Li
出处
期刊:Journal of Ethnopharmacology [Elsevier]
卷期号:319: 117191-117191 被引量:3
标识
DOI:10.1016/j.jep.2023.117191
摘要

Gleditsiae sinensis fructus Pills (GF) is a famous classical prescription, that is regularly combined with Jujubae fructus (JF) for the treatment of chronic bronchitis (CB) in the clinic. While the clinical efficacy of this combination prescription is clearly established, the active ingredients and molecular mechanisms remain unclear.To elucidate the mechanisms of action of Gleditsiae sinensis fructus Pills combined with Jujubae fructus (GF&JF) against CB based on network pharmacology and experimental verification.The potential targets of GF&JF involved in therapeutic activity against CB were predicted based on network pharmacology and an "ingredients-targets" network constructed. The Metascape database was used for Module, GO functional and KEGG signaling pathway enrichment analyses of potential targets. Molecular docking was applied to simulate the binding activities of key candidate active ingredients to core targets. For experimental verification, a CB model was established through smoking and nasal cavity drip of lipopolysaccharide. Related inflammatory factors, including TNF-α, TGF-β, IL-6 and IL-8 in serum, and IL-4 IL-8, IFN-γ and IL-10 in bronchoalveolar lavage fluid (BALF), were detected using ELISA. Hematoxylin and eosin (H&E) and Masson staining were performed to observe pathological changes in lung and tracheal tissue. The expression of related proteins and mRNAs in the lung tissue were detected using immunohistochemistry (IHC), quantitative real-time PCR, and western blot.In network pharmacology, 36 common targets of GF&JF for CB were screened and the key targets and main signaling pathways identified. The active ingredients quercetin and stigmasterol in GF&JF had more targets for CB, which displayed good binding activity to IL-6, VEGFA, and EGFR, as established from molecular docking results. In vivo, GF&JF effectively inhibit the inflammatory response in CB mice and improved pathological changes in lung and tracheal tissue. In terms of the key proteins of the AGE-RAGE signaling pathway, GF&JF induced significant down-regulation of IL-6, ICAM-1, VCAM-1, EGFR, CASPASE-3, AGEs and RAGE proteins in lung tissue as well as mRNA expression of IL-6, ICAM-1, VCAM-1, EGFR, AGEs and RAGE.The GF&JF combination exerts a good therapeutic effect in CB model mice, which may be attributed to inhibition of the inflammatory response as well as regulation on the expression of AGE-RAGE signaling pathway. In addition, quercetin and stigmasterol appear to be the main active ingredients of GF&JF in the treatment of CB.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
夏来应助科研通管家采纳,获得10
1秒前
思源应助科研通管家采纳,获得30
1秒前
1秒前
核桃发布了新的文献求助10
7秒前
情怀应助哈哈酱采纳,获得10
10秒前
传奇3应助河马采纳,获得30
11秒前
諭夏完成签到,获得积分20
17秒前
20秒前
24秒前
凡`发布了新的文献求助10
27秒前
pietro发布了新的文献求助10
27秒前
shuangshi1010发布了新的文献求助10
32秒前
友好的季节完成签到,获得积分10
37秒前
shuke发布了新的文献求助50
41秒前
42秒前
疯狂的师发布了新的文献求助10
44秒前
MyAI应助諭夏采纳,获得10
44秒前
yo发布了新的文献求助10
49秒前
指导灰发布了新的文献求助30
53秒前
李健的粉丝团团长应助CATH采纳,获得10
56秒前
orixero应助无问采纳,获得10
58秒前
1分钟前
1分钟前
1分钟前
1分钟前
疯狂的师发布了新的文献求助30
1分钟前
yo完成签到,获得积分10
1分钟前
shuangshi1010完成签到,获得积分10
1分钟前
CATH发布了新的文献求助10
1分钟前
1分钟前
zzw完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
1分钟前
我是老大应助zZ采纳,获得10
1分钟前
1分钟前
领导范儿应助张张采纳,获得10
1分钟前
无问发布了新的文献求助10
1分钟前
ame1120完成签到,获得积分10
1分钟前
疯狂的师发布了新的文献求助10
1分钟前
高分求助中
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
Retention of title in secured transactions law from a creditor's perspective: A comparative analysis of selected (non-)functional approaches 500
"Sixth plenary session of the Eighth Central Committee of the Communist Party of China" 400
New China Forges Ahead: Important Documents of the Third Session of the First National Committee of the Chinese People's Political Consultative Conference 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3056378
求助须知:如何正确求助?哪些是违规求助? 2712952
关于积分的说明 7433910
捐赠科研通 2357930
什么是DOI,文献DOI怎么找? 1249173
科研通“疑难数据库(出版商)”最低求助积分说明 606972
版权声明 596195