Enhanced long-term advanced denitrogenation from nitrate wastewater by anammox consortia: Dissimilatory nitrate reduction to ammonium (DNRA) coupling with anammox in an upflow biofilter reactor equipped with EDTA-2Na/Fe(II) ratio and pH control

• The Fe(II)-dependent DNRA-anammox process was first developed in an anammox reactor. • Candidatus Kuenenia was the dominant microbe in nitrate wastewater treatment system. • Visual MINTEQ analysis was conducted to estimate the predominant speciation. • Kinetic simulation was applied to describe the EDTA inhibition and pH dependency. • A TNRR of 0.23 ± 0.01 kg-N/m 3 /d was maintained for 60 days. A long-term experiment in an anaerobic ammonium oxidation (anammox) reactor showed that anammox consortia could perform a stable and efficient Fe(II)-dependent dissimilatory nitrate reduction to ammonium (DNRA) coupled to the anammox (DNRA-anammox) process by controlling the EDTA-2Na/Fe(II) ratio and pH, with a total nitrogen removal rate (TNRR) of 0.23 ± 0.01 kg-N/m 3 /d. Anammox bacteria ( Candidatus Kuenenia) were the dominant and functional microbes in such a nitrate wastewater treatment system. Visual MINTEQ analysis showed that the EDTA-2Na/Fe(II) molar ratio affected the influent composition of Fe and EDTA species and hence nitrate removal, while pH influenced both nitrate removal and the coupling degree of the Fe(II)-dependent DNRA-anammox process due to its own physiology. The kinetic simulation results showed that excess EDTA-2Na imposed a competitive inhibition on the Fe(II)-dependent DNRA-anammox process, and the Bell-shaped (A), (B), (C) and Ratkowsky models could be used to explore the pH dependency of the Fe(II)-dependent DNRA-anammox process.