适体
化学
检出限
生物传感器
滚动圆复制
DNA
自愈水凝胶
线性范围
纳米颗粒
聚丙烯酰胺
组合化学
纳米技术
色谱法
聚合酶
分子生物学
生物化学
高分子化学
材料科学
生物
作者
Pian Wu,Shuang Li,Xiaosheng Ye,Baoan Ning,Jialei Bai,Yuan Peng,Lei Li,Tie Han,Huanying Zhou,Zhixian Gao,Ping Ding
标识
DOI:10.1016/j.aca.2020.08.004
摘要
Sensitive and reliable analytical methods for monitoring of microcystin-LR (MC-LR) are urgently necessary due to its great harm to human health and aquatic organisms. In this work, a novel Cu/Au/Pt trimetallic nanoparticles (Cu/Au/Pt TNs)-encapsulated DNA hydrogel was prepared for colorimetric detection of MC-LR. The Cu/Au/Pt TNs were captured and released with precise control by the target-responsive 3D DNA hydrogels, which combined dual advantages of the target responsive DNA hydrogel and Cu/Au/Pt TNs of enhanced peroxidase-like activity. The DNA hydrogel network was constructed by hybridizing MC-LR aptamer with two complementary DNA strands on linear polyacrylamide chains. As long as MC-LR presented, the aptamer competitively binds with the MC-LR, causing the hydrogel to dissolve and release the preloaded Cu/Au/Pt TNs which could catalyze the reaction between H2O2 and TMB to produce color changes. In view of this sensitive strategy, this Cu/Au/Pt TNs-encapsulated DNA hydrogel-based colorimetric biosensor can achieve quantitative determination of MC-LR. The results showed that as-proposed colorimetric biosensor could sensitively detect MC-LR with a linear range of 4.0-10000 ng L-1 and a detection limit of 3.0 ng L-1. This work proved that the sensor had great potential to be applied in MC-LR detection and also provided the opportunity to develop colorimetric biosensor for other targets using this target-responsive and signal-amplification strategy.
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