糖蛋白组学
糖肽
蛋白质组
串联质谱法
糖基化
人类蛋白质组计划
质谱法
蛋白质组学
生物信息学
糖蛋白
聚糖
化学
计算生物学
生物化学
色谱法
生物
基因
抗生素
作者
Zilu Ye,Yang Mao,Henrik Clausen,Sergey Y. Vakhrushev
出处
期刊:Nature Methods
[Springer Nature]
日期:2019-08-05
卷期号:16 (9): 902-910
被引量:79
标识
DOI:10.1038/s41592-019-0504-x
摘要
We report a liquid chromatography coupled to tandem mass spectrometry O-glycoproteomics strategy using data-independent acquisition (DIA) mode for direct analysis of O-glycoproteins. This approach enables characterization of glycopeptides and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries (though it does not allow for direct unambiguous glycosite identification). The method relies on a spectral library of O-glycopeptides; the Glyco-DIA library contains sublibraries obtained from human cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the 5 most common core1 O-glycan structures. Applying the Glyco-DIA library to human serum without enrichment for glycopeptides enabled us to identify and quantify 269 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins in a SingleShot analysis.
科研通智能强力驱动
Strongly Powered by AbleSci AI