A selective mitochondria-targeted fluorescent probe for imaging cysteine in drug-induced liver injury

化学 荧光 肝损伤 半胱氨酸 药品 线粒体 生物物理学 纳米技术 生物化学 药理学 医学 物理 量子力学 生物 材料科学
作者
Guixin Qin,Lingli Gao,Nan Yin,Mingxiu Wang,Yuting Wang,Jiali Tang,Jianhua Gong,Qingling Xu
出处
期刊:Talanta [Elsevier]
卷期号:282: 127056-127056
标识
DOI:10.1016/j.talanta.2024.127056
摘要

Cysteine (Cys) is involved in many physiological processes. It's challenging to detect Cys selectively as it has similar chemical structure with other biothiols such as homocysteine (Hcy) and glutathione (GSH). In this work, a novel fluorescence probe toward mitochondrial cysteine, HPXI-6C, has been developed by employing carbonate as a new recognizing unit and hemicyanine as a chromophore. HPXI-6C exhibits a high selectivity to Cys over hydrogen sulfide, homocysteine and glutathione. The limit of detection toward Cys was determined to be 42 nM. HPXI-6C can localize in mitochondria and produce strong fluorescence peaked at 725 nm in response to Cys in tumor cells. The uptake and generation pathways of Cys in acetaminophen hepatotoxicity cells was revealed by using HPXI-6C. HPXI-6C has been successfully applied in imaging of Cys in drug-induced liver injury in vivo. The research demonstrated that HPXI-6C is powerful in monitoring Cys and is conducive to the early diagnosis of drug-induced liver injury diseases.
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