Fast and versatile analysis of liposome encapsulation efficiency by nanoParticle exclusion chromatography

脂质体 化学 色谱法 药品 高效液相色谱法 药物输送 体内分布 药物 剂型 活性成分 药理学 体外 有机化学 生物化学 医学
作者
Juan Bian,James Girotti,Yuchen Fan,Elizabeth S. Levy,Nanzhi Zang,Vijay A. Sethuraman,Ponien Kou,Kelly Zhang,Jason Gruenhagen,Jessica Lin
出处
期刊:Journal of Chromatography A [Elsevier]
卷期号:1662: 462688-462688 被引量:38
标识
DOI:10.1016/j.chroma.2021.462688
摘要

Liposomes are an attractive drug delivery platform for a wide variety of pharmaceutical molecules. Encapsulation efficiency, which refers to the amount of drug contained inside liposomes compared with the total amount of drug, is a critical quality attribute of liposome products, as the free drug in a liposomal formulation may cause toxicity or undesired biodistribution. The determination of encapsulation efficiency requires the measurement of at least two of the three drug populations: total drug, encapsulated drug and free drug. However, direct measurement of the encapsulated drug and free drug remains a challenging analytical task. Nanoparticle exclusion chromatography (nPEC), an emerging high-performance liquid chromatography (HPLC) technique, has shown great potential in separating and quantifying the free drug in liposomal formulations. In this study, nPEC was systematically evaluated for two representative liposomal formulations containing either hydrophilic or hydrophobic small molecule drugs. It is reported for the first time that the insoluble free drug suspended in the aqueous formulation can be directly measured by nPEC. This free drug in the suspension sample was quantified with excellent accuracy and precision. On the other hand, the total drug measurement from dissociated liposomes was confirmed by the benchmark methodology of reversed phase liquid chromatography (RPLC). The facile quantitation of free and total drug in the liposome formulation enables the fast and accurate determination of the encapsulation efficiency, which can be used to guide the formulation development and characterize the product quality.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
4u发布了新的文献求助10
1秒前
樊小雾完成签到,获得积分10
1秒前
CodeCraft应助tf采纳,获得10
1秒前
糖豆完成签到,获得积分20
1秒前
Justin发布了新的文献求助10
2秒前
ruirui关注了科研通微信公众号
2秒前
SciGPT应助上单马冬梅采纳,获得10
2秒前
英姑应助清新的初雪采纳,获得10
2秒前
2秒前
3秒前
3秒前
4秒前
活泼弼完成签到,获得积分10
5秒前
传奇3应助默默采纳,获得10
5秒前
科研通AI6.1应助囡囡采纳,获得10
5秒前
sa1t完成签到,获得积分20
5秒前
白白完成签到,获得积分10
5秒前
蓝莓橘子酱应助辻渃采纳,获得10
5秒前
成就糖豆完成签到,获得积分10
5秒前
6秒前
6秒前
繁星发布了新的文献求助10
6秒前
DHL完成签到,获得积分10
6秒前
7秒前
7秒前
香蕉初曼发布了新的文献求助10
7秒前
8秒前
8秒前
8秒前
8秒前
动听的聋五完成签到,获得积分10
8秒前
Zed plus完成签到,获得积分10
8秒前
Bminor完成签到,获得积分10
8秒前
汉堡包应助Justin采纳,获得10
8秒前
有趣的桃发布了新的文献求助10
9秒前
9秒前
hhh完成签到 ,获得积分10
9秒前
9秒前
星辰大海应助李琛璐采纳,获得10
10秒前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Handbook of pharmaceutical excipients, Ninth edition 5000
Aerospace Standards Index - 2026 ASIN2026 3000
Signals, Systems, and Signal Processing 610
Discrete-Time Signals and Systems 610
Research Methods for Business: A Skill Building Approach, 9th Edition 500
Social Work and Social Welfare: An Invitation(7th Edition) 410
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 纳米技术 有机化学 物理 生物化学 化学工程 计算机科学 复合材料 内科学 催化作用 光电子学 物理化学 电极 冶金 遗传学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 6054599
求助须知:如何正确求助?哪些是违规求助? 7879152
关于积分的说明 16283648
捐赠科研通 5199861
什么是DOI,文献DOI怎么找? 2782391
邀请新用户注册赠送积分活动 1765143
关于科研通互助平台的介绍 1646451