神经化学
神经科学
神经活动
钙显像
生物
化学
钙
有机化学
作者
Chunyang Dong,Yufeng Zheng,Kiran Long-Iyer,Emily C. Wright,Yulong Li,Lin Tian
出处
期刊:Annual Review of Neuroscience
[Annual Reviews]
日期:2022-03-22
卷期号:45 (1): 273-294
被引量:54
标识
DOI:10.1146/annurev-neuro-110520-031137
摘要
Recent advances in fluorescence imaging permit large-scale recording of neural activity and dynamics of neurochemical release with unprecedented resolution in behaving animals. Calcium imaging with highly optimized genetically encoded indicators provides a mesoscopic view of neural activity from genetically defined populations at cellular and subcellular resolutions. Rigorously improved voltage sensors and microscopy allow for robust spike imaging of populational neurons in various brain regions. In addition, recent protein engineering efforts in the past few years have led to the development of sensors for neurotransmitters and neuromodulators. Here, we discuss the development and applications of these genetically encoded fluorescent indicators in reporting neural activity in response to various behaviors in different biological systems as well as in drug discovery. We also report a simple model to guide sensor selection and optimization.
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