生物
MYB公司
类黄酮生物合成
次生代谢
非生物胁迫
转录因子
脱落酸
花青素
植物生理学
生物化学
耐旱性
基因
转基因
细胞生物学
类黄酮
基因表达
黄芩
植物
查尔酮合酶
拟南芥
烟草
转基因作物
作者
Yuan Yuan,Linjie Qi,Jian Yang,Chong Wu,Yun-Jun Liu,Luqi Huang
标识
DOI:10.1007/s11240-014-0650-x
摘要
R2R3-MYB proteins are involved in the primary and secondary metabolism, developmental processes and the responses to biotic and abiotic stresses. Little is known about the functions of R2R3-MYB proteins in Scutellaria baicalensis Georgi which is a traditional Chinese medicinal plants. In this study, the function of a S. baicalensis R2R3-MYB protein, SbMYB8, was investigated. SbMYB8 had similar expression pattern with SbC4H and SbCHS in ABA-treated S. baicalensis, indicating that SbMYB8 might be involved in the flavonoid metabolism. SbMYB8 protein could bind to the GmMYB92 BS3 sequence of SbCHS promoter region, regulating the expression of SbCHS. The SbMYB8 protein was localized to the nucleus where it activated transcription. The transgenic tobacco plants over-expressing SbMYB8 had higher caffeoylquinic acid contents, compared to that in wild type plants. Overexpression of SbMYB8 also changed the expression level of some flavonoid biosynthesis-related genes. It was found that overexpression of SbMYB8 can improve stress tolerance of transgenic plants, and can alter the activity and expression levels of some antioxidant enzymes. These results indicate that SbMYB8 plays important roles in flavonoid biosynthesis and stress tolerance of plant.
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