生物
衣壳
帽状体
向性
病毒学
病毒
梅克尔多元癌细胞病毒
细胞生物学
转化(遗传学)
成纤维细胞
组蛋白
细胞培养
分子生物学
遗传学
DNA
基因
梅克尔细胞癌
癌
作者
Lenka Horníková,Vojtěch Žíla,Hana Kolářová,Jitka Forstová
标识
DOI:10.1002/9780471729259.mc14f01s38
摘要
Mouse polyomavirus (MPyV) is a member of the Polyomaviridae family, which comprises non-enveloped tumorigenic viruses infecting various vertebrates including humans and causing different pathogenic responses in the infected organisms. Despite the variations in host tropism and pathogenicity, the structure of the virions of these viruses is similar. The capsid, with icosahedral symmetry (ø, 45 nm, T = 7d), is composed of a shell of 72 capsomeres of structural proteins, arranged around the nucleocore containing approximately 5-kbp-long circular dsDNA in complex with cellular histones. MPyV has been one of the most studied polyomaviruses and serves as a model virus for studies of the mechanisms of cell transformation and virus trafficking, and for use in nanotechnology. It can be propagated in primary mouse cells (e.g., in whole mouse embryo cells) or in mouse epithelial or fibroblast cell lines. In this unit, propagation, purification, quantification, and storage of MPyV virions are presented.
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