聚丙烯腈
脂肪酶
化学
腈
固定化酶
酯交换
生物柴油
膜
产量(工程)
有机化学
色谱法
酶
催化作用
聚合物
生物化学
材料科学
冶金
作者
Youran Li,Hanrong Wang,Jiawei Lu,Alex Chu,Liang Zhang,Zhongyang Ding,Sha Xu,Zhenghua Gu,Guiyang Shi
标识
DOI:10.1016/j.biortech.2018.11.075
摘要
The application of immobilized lipase in the enzymatic production of biodiesel has shown numerous advantages. In this study, surface of Polyacrylonitrile (PAN) hollow membrane was first modified using nitrile-click chemistry in order to fit for interaction with enzyme proteins. Then sodium alginate (SA) was introduced and the membrane was post-treated by CaCl2. When the prepared PAN-PEI-SA-CaCl2 was used for lipase immobilization, the protein loading was 36.90 mg/g, and the enzyme activity reached up to 54.47 U/g, which was 2.5 times as much as that of Novozym® 435. As a result, the constructed immobilized lipase obtained a maximum biodiesel yield of 78.5%, which was 2.4 times that of the Novozym® 435 in transesterification reactions. Moreover, the biodiesel yield decreased by only 11% after the immobilized enzyme was continuously used for 20 times. This study exhibits that this technic has broad application prospects in the field of conversion of biomass resources.
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