细胞器
丝状体
内质网
活体细胞成像
生物物理学
显微镜
膜
分辨率(逻辑)
细胞生物学
化学
纳米技术
细胞
生物
材料科学
肌动蛋白
计算机科学
光学
物理
人工智能
生物化学
作者
Hideo Takakura,Yongdeng Zhang,Roman S. Erdmann,Alexander D. Thompson,Yu Lin,Brian McNellis,Felix Rivera‐Molina,Shin‐nosuke Uno,Mako Kamiya,Yasuteru Urano,James E. Rothman,Joerg Bewersdorf,Alanna Schepartz,Derek Toomre
摘要
Imaging cellular structures and organelles in living cells by long time-lapse super-resolution microscopy is challenging, as it requires dense labeling, bright and highly photostable dyes, and non-toxic conditions. We introduce a set of high-density, environment-sensitive (HIDE) membrane probes, based on the membrane-permeable silicon-rhodamine dye HMSiR, that assemble in situ and enable long time-lapse, live-cell nanoscopy of discrete cellular structures and organelles with high spatiotemporal resolution. HIDE-enabled nanoscopy movies span tens of minutes, whereas movies obtained with labeled proteins span tens of seconds. Our data reveal 2D dynamics of the mitochondria, plasma membrane and filopodia, and the 2D and 3D dynamics of the endoplasmic reticulum, in living cells. HIDE probes also facilitate acquisition of live-cell, two-color, super-resolution images, expanding the utility of nanoscopy to visualize dynamic processes and structures in living cells.
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