水杨酸
茉莉酸
生物
茉莉酸
茉莉酸甲酯
拟南芥
类黄酮生物合成
对抗
植物
发病相关蛋白
拟南芥
植物激素
防御机制
类黄酮
植物对草食的防御
基因表达
基因
生物化学
转录组
抗氧化剂
受体
突变体
作者
Chhana Ullah,Axel Schmidt,Michael Reichelt,Chung‐Jui Tsai,Jonathan Gershenzon
摘要
Salicylic acid (SA) and jasmonic acid (JA) often play distinct roles in plant defence against pathogens. Research from Arabidopsis thaliana has established that SA- and JA-mediated defences are more effective against biotrophs and necrotrophs, respectively. These two hormones often interact antagonistically in response to particular attackers, with the induction of one leading to suppression of the other. Here, we report a contrasting pattern in the woody perennial Populus: positive SA-JA interplay. Using genetically engineered high SA lines of black poplar and wild-type lines after exogenous hormone application, we quantified SA and JA metabolites, signalling gene transcripts, antifungal flavonoids and resistance to rust (Melampsora larici-populina). Salicylic acid and JA metabolites were induced concurrently upon rust infection in poplar genotypes with varying resistance levels. Analysis of SA-hyperaccumulating transgenic poplar lines showed increased jasmonate levels, elevated flavonoid content and enhanced rust resistance, but no discernible reduction in growth. Exogenous application of either SA or JA triggered the accumulation of the other hormone. Expression of pathogenesis-related (PR) genes, frequently used as markers for SA signalling, was not correlated with SA content, but rather activated in proportion to pathogen infection. We conclude that SA and JA pathways interact positively in poplar resulting in the accumulation of flavonoid phytoalexins.
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