Platelet Cryopreservation Using a Reduced Dimethyl Sulfoxide Concentration and Second-Messenger Effectors as Cryopreserving Solution

低温保存 血小板 低温保护剂 二甲基亚砜 化学 血管性血友病因子 男科 纤维蛋白原 血小板活化 生物化学 免疫学 生物 细胞生物学 医学 胚胎 有机化学
作者
Marı́a Luisa Lozano,José Rivera,Javier Corral,Rocío Gónzález‐Conejero,Vicente Vicente
出处
期刊:Cryobiology [Elsevier]
卷期号:39 (1): 1-12 被引量:18
标识
DOI:10.1006/cryo.1999.2184
摘要

Cryopreservation of platelets is of great interest since it could extend to years the shelf life of therapeutic platelet concentrates (PCs) and facilitate stockpiling and inventory control in blood banking. We have compared the cryopreservation of PCs by the standard method using 6% Me2SO as cryoprotectant with the method of freezing employing low concentrations of Me2SO (2%) plus ThromboSol, a mixture of second-messenger effectors that protect platelets from cold damage. PC pools were treated either with 6% Me2SO or with ThromboSol and 2% Me2SO and then placed directly in a −80°C freezer or in the vapor phase of a liquid nitrogen freezer (−120°C). After storage for 1 week or for 3 months, samples were removed, thawed, and analyzed. Measurements included cell recovery, biochemical parameters, membrane glycoproteins (GPs), platelet aggregation, and binding of radiolabeled von Willebrand factor (vWF) and fibrinogen. PCs cryopreserved with ThromboSol and 2% Me2SO displayed a platelet recovery (90%) equivalent to those frozen with 6% Me2SO. Following either cryopreservation procedure, platelets showed increased surface expression of P-selectin and moderate loss of GP Ibα in comparison to fresh platelets. The aggregatory response to ristocetin and the binding of vWF were similar in platelets frozen by either procedure. Finally, both methods promoted comparable impairment of the reactivity of platelets to thrombin, aggregation and binding of fibrinogen and vWF, compared to that of fresh platelets. In summary, cryopreservation of PCs using reduced Me2SO concentration and ThromboSol yields platelets with in vitro functional characteristics equivalent to those of cells frozen with the conventional method using 6% Me2SO.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Foremelon发布了新的文献求助10
1秒前
1秒前
山石二道疤完成签到,获得积分10
2秒前
3秒前
浣熊小呆发布了新的文献求助10
4秒前
pigzhu发布了新的文献求助10
4秒前
5秒前
5秒前
莫莫完成签到,获得积分10
5秒前
6秒前
mbf发布了新的文献求助10
7秒前
7秒前
8秒前
莫莫发布了新的文献求助10
8秒前
9秒前
Luo发布了新的文献求助10
10秒前
10秒前
完美世界应助LH采纳,获得10
13秒前
负责流口水完成签到,获得积分10
13秒前
13秒前
huohuo完成签到,获得积分10
14秒前
啦啦啦发布了新的文献求助10
14秒前
echo驳回了bkagyin应助
14秒前
Drorme发布了新的文献求助10
15秒前
Dean完成签到 ,获得积分10
16秒前
外向易形发布了新的文献求助10
16秒前
学术蟑螂完成签到,获得积分10
18秒前
19秒前
才下眉头完成签到,获得积分10
21秒前
21秒前
米子完成签到,获得积分10
21秒前
21秒前
23秒前
Zz完成签到 ,获得积分10
23秒前
25秒前
Foremelon发布了新的文献求助10
26秒前
guantlv发布了新的文献求助10
26秒前
27秒前
小捣腾发布了新的文献求助10
27秒前
27秒前
高分求助中
Evolution 10000
Sustainability in Tides Chemistry 2800
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
An Introduction to Geographical and Urban Economics: A Spiky World Book by Charles van Marrewijk, Harry Garretsen, and Steven Brakman 600
Diagnostic immunohistochemistry : theranostic and genomic applications 6th Edition 500
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3153169
求助须知:如何正确求助?哪些是违规求助? 2804457
关于积分的说明 7859169
捐赠科研通 2462280
什么是DOI,文献DOI怎么找? 1310725
科研通“疑难数据库(出版商)”最低求助积分说明 629377
版权声明 601794