低温保存
血小板
低温保护剂
二甲基亚砜
化学
血管性血友病因子
男科
纤维蛋白原
血小板活化
生物化学
免疫学
生物
细胞生物学
医学
胚胎
有机化学
作者
Marı́a Luisa Lozano,José Rivera,Javier Corral,Rocío Gónzález‐Conejero,Vicente Vicente
出处
期刊:Cryobiology
[Elsevier]
日期:1999-08-01
卷期号:39 (1): 1-12
被引量:18
标识
DOI:10.1006/cryo.1999.2184
摘要
Cryopreservation of platelets is of great interest since it could extend to years the shelf life of therapeutic platelet concentrates (PCs) and facilitate stockpiling and inventory control in blood banking. We have compared the cryopreservation of PCs by the standard method using 6% Me2SO as cryoprotectant with the method of freezing employing low concentrations of Me2SO (2%) plus ThromboSol, a mixture of second-messenger effectors that protect platelets from cold damage. PC pools were treated either with 6% Me2SO or with ThromboSol and 2% Me2SO and then placed directly in a −80°C freezer or in the vapor phase of a liquid nitrogen freezer (−120°C). After storage for 1 week or for 3 months, samples were removed, thawed, and analyzed. Measurements included cell recovery, biochemical parameters, membrane glycoproteins (GPs), platelet aggregation, and binding of radiolabeled von Willebrand factor (vWF) and fibrinogen. PCs cryopreserved with ThromboSol and 2% Me2SO displayed a platelet recovery (90%) equivalent to those frozen with 6% Me2SO. Following either cryopreservation procedure, platelets showed increased surface expression of P-selectin and moderate loss of GP Ibα in comparison to fresh platelets. The aggregatory response to ristocetin and the binding of vWF were similar in platelets frozen by either procedure. Finally, both methods promoted comparable impairment of the reactivity of platelets to thrombin, aggregation and binding of fibrinogen and vWF, compared to that of fresh platelets. In summary, cryopreservation of PCs using reduced Me2SO concentration and ThromboSol yields platelets with in vitro functional characteristics equivalent to those of cells frozen with the conventional method using 6% Me2SO.
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