细胞凋亡
标记法
转染
末端脱氧核苷酸转移酶
内皮干细胞
电泳迁移率测定
NF-κB
生物
细胞生物学
分子生物学
癌症研究
细胞
NFKB1型
细胞培养
生物化学
体外
基因表达
转录因子
基因
遗传学
作者
See‐Tarn Woon,Sandy Hung,Derek C.F. Wu,Mary Ann Schooltink,R. M. Sutherland,Bruce C. Baguley,Qi Chen,Lawrence W. Chamley,Lai-Ming Ching
出处
期刊:PubMed
[National Institutes of Health]
日期:2007-03-14
卷期号:27 (1A): 327-34
被引量:11
摘要
DMXAA (5,6-dimethylxanthenone-4-acetic acid; AS1404), a vascular disrupting agent currently in clinical trials, induces tumour endothelial cell apoptosis in vivo in mice and in cancer patients. DMXAA activates NF-kappaB in many different cell types. In this study, whether DMXAA-induced endothelial cell apoptosis was NF-kappaB dependent was determined.HUVEC endothelial and T24 endothelial-like cells were treated with DMXAA and apoptosis was measured by terminal deoxynucleotidyl transferase biotin-dUTP nick end labelling (TUNEL). NF-kappaB activation was measured by electrophoretic mobility shift assays (EMSA). T24 cells were transfected with IkappaBalphaM, a mutant form of the IkappaBalpha gene which cannot be phosphorylated and degraded, hence preventing NF-kappaB expression.No NF-kappaB up-regulation was detected in apoptotic HUVEC treated with DMXAA. The IkappaBalphaM-transfected T24 cells showed similar apoptotic responses to those of parental cells.The DMXAA-induced apoptosis is neither mediated by, nor inhibited by, the expression of the NF-kappaB pathway.
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