Combining a hybrid chip and tube microfluidic system with fluorescent molecularly imprinted polymer (MIP) core–shell particles for the derivatisation, extraction, and detection of peptides with N-terminating phosphorylated tyrosine

A microfluidic ratiometric detection scheme using silica nanoparticles coated with a “light-up” fluorescent molecularly imprinted polymer allows selective rapid assaying of pY -terminating peptides after in-tube/on-chip derivatisation and extraction.