Acupuncture ameliorates depression-like behavior of poststroke depression model rats through the regulation of gut microbiota and NLRP3 inflammasome in the colon

炎症体 行为绝望测验 肠道菌群 针灸科 开阔地 肠-脑轴 医学 益生菌 内分泌学 内科学 炎症 生物 免疫学 替代医学 海马体 病理 遗传学 细菌 抗抑郁药
作者
Wa Cai,Xi-Fang Wei,Jing-Ruo Zhang,Larissa Tao,Dong Li,Kun Zhang,Weidong Shen
出处
期刊:Neuroreport [Ovid Technologies (Wolters Kluwer)]
卷期号:35 (14): 883-894
标识
DOI:10.1097/wnr.0000000000002076
摘要

This study was conducted to examine the effects of acupuncture on gut microbiota and expression of NLRP3 inflammasome in the colon in poststroke depression (PSD) model rats. Sprague–Dawley male rats were randomized into four groups: sham surgery group, poststroke depression group, acupuncture group, and probiotics group. Acupuncture therapy at Baihui (GV20), Shenting (GV24), bilateral Zusanli (ST36) acupoints in the acupuncture group and probiotic gavage therapy in the probiotics group were performed once per day for 2 weeks. Behaviors of depression were assessed by using weight measurements, sucrose preference test, open field test, and forced swimming test. Histopathological alterations in the colon were determined by hematoxylin–eosin staining, the expression of NLRP3/ASC/caspase-1 pathway-related proteins was analyzed by western blotting. Serum levels of IL-1β and IL-18 were derived from ELISA. The 16S rRNA gene sequencing was performed to examine and analyze the differences of gut microbiota of rats among all groups. Acupuncture was effective to increase weight and ameliorate depressive-like behaviors in PSD rats. Acupuncture increased the diversity of gut microbiota, upregulated the abundance of Bifidobacteriaceae and Lactobacillaceae, and decreased the relative abundance of Peptostreptococcaceae, Rikenellaceae, Eggerthellaceae, and Streptococcaceae at family level. Acupuncture effectively improved the pathological changes in the colon. Meanwhile, acupuncture reduced NLRP3, ASC, caspase-1 protein expressions in the colon, and serum levels of IL-18 and IL-1β. Acupuncture may reduce depressive-like behaviors of PSD by regulating the gut microbiota and suppressing hyperactivation of NLRP3 inflammasome in the colon. Microbiota–gut–brain axis may be an effective target pathway for acupuncture treatment of PSD.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Ste完成签到,获得积分10
1秒前
1秒前
壮观半鬼完成签到,获得积分10
2秒前
chen完成签到,获得积分10
3秒前
兔子爱吃胡萝卜完成签到,获得积分10
4秒前
圆圆姐姐发布了新的文献求助10
5秒前
独特天问发布了新的文献求助10
5秒前
lll完成签到,获得积分10
6秒前
小困完成签到,获得积分10
7秒前
7秒前
852应助小白菜采纳,获得10
7秒前
CipherSage应助Wht采纳,获得10
10秒前
下次一定发布了新的文献求助10
10秒前
大模型应助FAPI采纳,获得10
11秒前
戴好头盔搞科研完成签到,获得积分10
11秒前
在水一方应助温柔若颜采纳,获得10
12秒前
12秒前
清秀的惜萱完成签到,获得积分20
12秒前
12秒前
12秒前
科研通AI2S应助gar采纳,获得30
13秒前
张恒完成签到,获得积分10
15秒前
独特天问完成签到,获得积分10
15秒前
guofzh完成签到,获得积分10
17秒前
不配.应助shufessm采纳,获得10
17秒前
叙温雨发布了新的文献求助10
17秒前
到家了完成签到,获得积分20
18秒前
20秒前
哭泣的幻翠完成签到 ,获得积分10
21秒前
22秒前
壮观半鬼发布了新的文献求助10
23秒前
23秒前
Jasper应助小媛采纳,获得10
24秒前
可爱的函函应助蒸蒸日上采纳,获得10
24秒前
blueisthe完成签到 ,获得积分10
26秒前
科研通AI2S应助到家了采纳,获得10
26秒前
26秒前
27秒前
long完成签到 ,获得积分10
27秒前
顾瞻完成签到,获得积分10
28秒前
高分求助中
Evolution 10000
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
Diagnostic immunohistochemistry : theranostic and genomic applications 6th Edition 500
Chen Hansheng: China’s Last Romantic Revolutionary 500
China's Relations With Japan 1945-83: The Role of Liao Chengzhi 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3149266
求助须知:如何正确求助?哪些是违规求助? 2800354
关于积分的说明 7839707
捐赠科研通 2457979
什么是DOI,文献DOI怎么找? 1308158
科研通“疑难数据库(出版商)”最低求助积分说明 628456
版权声明 601706