微泡
外体
干细胞
基质凝胶
细胞生物学
微流控
癌症干细胞
化学
细胞
纳米技术
生物
材料科学
生物化学
小RNA
基因
作者
Xingyue Peng,Pengxiang Su,Yaxin Guo,Jing Zhang,Linghan Peng,Rongrong Zhang
标识
DOI:10.3390/ijms241713419
摘要
Cell-to-cell communication must occur through molecular transport in the intercellular fluid space. Nanoparticles, such as exosomes, diffuse or move more slowly in fluids than small molecules. To find a microfluidic technology for real-time exosome experiments on intercellular communication between living cells, we use the microfluidic culture dish’s quaternary ultra-slow microcirculation flow field to accumulate nanoparticles in a specific area. Taking stem cell–tumor cell interaction as an example, the ultra-slow microcirculatory flow field controls stem cell exosomes to interfere with tumor cells remotely. Under static coculture conditions (without microfluidics), the tumor cells near stem cells (<200 µm) show quick breaking through from its Matrigel drop to meet stem cells, but this ‘breaking through’ quickly disappears with increasing distance. In programmed ultra-slow microcirculation, stem cells induce tumor cells 5000 μm far at the site of exosome deposition (according to nanoparticle simulations). After 14 days of programmed coculture, the glomeration and migration of tumor cells were observed in the exosome deposition area. This example shows that the ultra-slow microcirculation of the microfluidic culture dish has good prospects in quantitative experiments to study exosome communication between living cells and drug development of cancer metastasis.
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