亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

N-methyladenosine profiling reveals that Xuefu Zhuyu decoction upregulates METTL14 and BDNF in a rat model of traumatic brain injury

小桶 汤剂 N6-甲基腺苷 神经保护 创伤性脑损伤 莫里斯水上航行任务 神经营养因子 医学 脑源性神经营养因子 药理学 基因表达 生物 传统医学 基因 海马体 内科学 生物化学 精神科 基因本体论 甲基转移酶 甲基化 受体
作者
Dandan Feng,Pengfei Li,Wei Xiao,Zhuan Pei,Peishun Chen,Mingrui Hu,Zhaoyu Yang,Teng Li,Zian Xia,Hanjin Cui,Haigang Li,Qing Huang,Wei Zhang,Tao Tang,Yang Wang
出处
期刊:Journal of Ethnopharmacology [Elsevier]
卷期号:317: 116823-116823 被引量:7
标识
DOI:10.1016/j.jep.2023.116823
摘要

The traditional Chinese herbal formula Xuefu Zhuyu decoction (XFZYD) is a classic formula in the category of invigorating blood circulation and resolving blood stasis. It has been proven to improve the neurological and ethological prognosis of traumatic brain injury. XFZYD promotes synaptic and axonal regeneration after traumatic brain injury, which is functionally modulated by the N6-methyladenosine (m6A) modification of RNA. However, the epigenetic effects of XFZYD on m6A modification remain unknown.To explore how XFZYD protects against traumatic brain injury induced by controlled cortical impact (CCI) injury by altering RNA m6A modification.The modified neurological severity scoring and Morris water maze were performed to evaluate the neuroprotective effects of XFZYD for 14 days and screen the dose. Then, dot blot, western blotting, and methylated RNA immunoprecipitation sequencing (MeRIP-Seq) were used to explore changes in RNA m6A modification in the perilesional cortex. The Metascape platform was used to analyze the Gene Ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome pathway of the differential m6A-tagged genes. Furthermore, MeRIP-qPCR was conducted to quantify differences in the hub differential m6A modification gene brain-derived neurotrophic factor (Bdnf).XFZYD significantly ameliorated the neurological deficits, spatial learning, and memory impairments in rats post-CCI on day 14. XFZYD enhanced the m6A level, and the expression of METTL14 and YTHDC2 in the perilesional cortex of CCI rats. In all three groups, the 3'-untranslated regions and coding sequence were primarily enriched for m6A peaks. XFZYD reversed the increased proportion of 3'-untranslated regions, and the decreased proportion of coding sequence and 5'-untranslated regions post-CCI. Moreover, XFZYD markedly downregulated 41 elevated m6A-tagged transcripts and upregulated 119 decreased m6A-tagged transcripts following CCI. Gene ontology and KEGG pathway analysis revealed that XFZYD-regulated m6A-tagged transcripts were predominantly enriched in synapse assembly, synaptic plasticity, learning or memory, and MAPK signaling pathway. Then, the hub-regulated m6A-tagged gene BDNF was identified. Both the m6A methylation level and the protein level of BDNF were ascended by XFZYD treatment.XFZYD improves neurological deficits, spatial learning and memory impairments in rats post-TBI probably through increasing the expression of METTL14 and BDNF in the cortex. Our study highlights a novel post-transcriptional regulation mechanism mediated by herbal medicine for traumatic brain injury treatment.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
粽子发布了新的文献求助10
刚刚
FashionBoy应助Fox采纳,获得30
2秒前
领导范儿应助科研通管家采纳,获得10
3秒前
科研通AI2S应助科研通管家采纳,获得10
3秒前
科研通AI2S应助科研通管家采纳,获得10
3秒前
不易发布了新的文献求助10
3秒前
124332发布了新的文献求助10
6秒前
风中的惊蛰完成签到,获得积分10
8秒前
姆姆没买完成签到 ,获得积分10
18秒前
19秒前
21秒前
ni发布了新的文献求助10
22秒前
丝瓜发布了新的文献求助10
24秒前
31秒前
领导范儿应助ni采纳,获得10
35秒前
Arjun应助124332采纳,获得80
36秒前
43秒前
科研通AI2S应助sppig采纳,获得10
1分钟前
爱静静应助will采纳,获得30
1分钟前
行走完成签到,获得积分10
1分钟前
星辰大海应助Summer采纳,获得10
1分钟前
1分钟前
1分钟前
2分钟前
科研通AI2S应助科研通管家采纳,获得10
2分钟前
不易发布了新的文献求助10
2分钟前
dawnfrf完成签到,获得积分10
2分钟前
CodeCraft应助axiao采纳,获得10
2分钟前
ZHErain完成签到,获得积分10
2分钟前
2分钟前
2分钟前
田様应助124332采纳,获得150
2分钟前
axiao发布了新的文献求助10
2分钟前
Nacy发布了新的文献求助10
2分钟前
Billy应助啦啦啦啦啦采纳,获得30
2分钟前
2分钟前
香蕉觅云应助ZHErain采纳,获得10
2分钟前
2分钟前
StayGolDay完成签到,获得积分10
2分钟前
2分钟前
高分求助中
Rock-Forming Minerals, Volume 3C, Sheet Silicates: Clay Minerals 2000
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Very-high-order BVD Schemes Using β-variable THINC Method 930
The Vladimirov Diaries [by Peter Vladimirov] 600
Development of general formulas for bolted flanges, by E.O. Waters [and others] 600
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3265467
求助须知:如何正确求助?哪些是违规求助? 2905482
关于积分的说明 8333920
捐赠科研通 2575775
什么是DOI,文献DOI怎么找? 1400130
科研通“疑难数据库(出版商)”最低求助积分说明 654702
邀请新用户注册赠送积分活动 633525