化学
核酸酶
核酸内切酶
DNA
复式(建筑)
劈开
计算生物学
生物物理学
分子生物学
生物化学
生物
作者
Yueying Li,Su Jiang,Tingting Pan,Yanbo Wang,Chun‐yang Zhang
出处
期刊:Talanta
[Elsevier]
日期:2024-09-10
卷期号:281: 126863-126863
标识
DOI:10.1016/j.talanta.2024.126863
摘要
Flap endonuclease 1 (FEN1) is a structure-specific nuclease that can specially identify and cleave 5' flap of branched duplex DNA, and it plays a critical role in DNA metabolic pathways and human diseases. Herein, we propose a simple "mix-and-detection" strategy for sensitive measurement of human cellular FEN1 on basis of template-free amplification. We design a dumbbell probe with 5' flap as a substrate of FEN1 and a NH
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