Over the last few years, electroanalysis has made significant advancements, particularly in developing electrochemical sensors. Electrochemical sensors generally include emerging Photoelectrochemical and Electrochemiluminescence sensors, which combine optical techniques and traditional electrochemical bio/non-biosensors. Numerous EC-detecting methods have also been designed for commercial applications to detect biological and non-biological markers for various diseases. Analytical applications have recently focused significantly on one of the novel nanomaterials, the MXene. This material is being extensively investigated for applications in electrochemical sensors due to its unique mechanical, electronic, optical, active functional groups and thermal characteristics. This study extensively discusses the salient features of MXene-based electrochemical sensors, photoelectrochemical sensors, enzyme-based biosensors, immunosensors, aptasensors, electrochemiluminescence sensors, and electrochemical non-biosensors. In addition, their performance in detecting various substances and contaminants is thoroughly discussed. Furthermore, the challenges and prospects the MXene-based electrochemical sensors are elaborated.