电极
介电谱
检出限
线性范围
材料科学
分析化学(期刊)
微分脉冲伏安法
重复性
电化学
电化学气体传感器
循环伏安法
再现性
化学
色谱法
物理化学
作者
Yuliang Zhao,Hongyu Zhang,Yang Li,Xiaoai Wang,Liang Zhao,Jianghong Xu,Zhikun Zhan,Guanglie Zhang,Wen J. Li
出处
期刊:Biosensors
[MDPI AG]
日期:2022-10-21
卷期号:12 (10): 902-902
被引量:2
摘要
An electrochemical HbA1c sensor with high sensitivity and good specificity is proposed based on the electrochemical immune principle. The reproducibility and conductivity of the electrode are improved by depositing gold nanoparticles (AuNPs) on the surface of the screen-printed electrode (SPE). The HbA1c antibodies are immobilized on the surface of the modified electrode by adsorption to capture the HbA1c in the sample. The hindering effect of HbA1c on the electrode transfer reaction was exploited as the HbA1c detection mechanism. The electrode's properties were characterized by electrochemical impedance spectroscopy (EIS), and the measurement properties of the electrode were analyzed using differential pulse voltammetry (DPV) and cyclic voltammetry (CV). The experimental results show that the peak current signal of the electrochemical immunosensor produced a linear response to HbA1c in the concentration range of 20-200 μg/mL, a linear relationship coefficient of 0.9812, a detection limit of 15.5 µg/mL, and a sensitivity of 0.0938 µA/µg·mL-1. The sensor delivered satisfactory repeatability, stability, and anti-interference performance. Due to its small size, high sensitivity, and wide linear detection range, it is expected to play a significant role in managing diabetes at home.
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