化学
双层
小泡
铕
磷脂
发光
脂质双层
脂质体
离子
磷脂酰胆碱
光化学
分析化学(期刊)
膜
色谱法
有机化学
生物化学
物理
光电子学
作者
Beth M. Cader,William DeW. Horrocks
标识
DOI:10.1016/0301-4622(88)85038-5
摘要
Laser-induced europium(III) luminescence spectroscopy was used to investigate the formation and integrity of phospholipid bilayer vesicles produced by ultrasonication and detergent dialysis. Eu(III) ion interactions with these model biological membrane systems were explored. 7F0 → 5D0 spectral and excited-state lifetime data reveal two distinct, temperature-dependent binding sites, one involving a weak, 'superficial' interaction with the phosphate moiety of the phosphatidylcholine head group, the other involving a more tightly bound ion in a relatively dehydrated region of the head group. This latter 'sequestered' species appeared only at temperatures equal to or below that of the gel → liquid-crystalline phase transition. Systems containing various amounts of cholesterol showed a decrease in the formation of the sequestered species, indicative of a decrease in ion permeability. The results of this study demonstrate that the Eu(III) luminescence technique is useful for detecting major phase alterations in phospholipid bilayer vesicles.
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