化学
胆固醇氧化酶
胆固醇
色谱法
过氧化氢
试剂
水解
苯酚
酶
校准曲线
过氧化物酶
生物化学
有机化学
检出限
作者
Charles C. Allain,Lucy S Poon,Cicely S G Chan,W. Richmond,Paul Fu
出处
期刊:Clinical Chemistry
[Oxford University Press]
日期:1974-04-01
卷期号:20 (4): 470-475
被引量:9176
标识
DOI:10.1093/clinchem/20.4.470
摘要
Abstract An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent. The method requires no prior treatment of sample and the calibration curve is linear to 600 mg/dl. Cholesterol esters are hydrolyzed to free cholesterol by cholesterol ester hydrolase (EC 3.1.1.13). The free cholesterol produced is oxidized by cholesterol oxidase to cholest-4-en-3-one with the simultaneous production of hydrogen peroxide, which oxidatively couples with 4-aminoantipyrine and phenol in the presence of peroxidase to yield a chromogen with maximum absorption at 500 nm. The method is reproducible, and the results correlate well with those obtained by automated Liebermann—Burchard procedures (AA-2 and SMA 12/60) and the method of Abell et al. The present method affords better specificity than those previously reported and has excellent precision.
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