Structural characterization of feruloyl oligosaccharides from spinach-leaf cell walls

Hydrolysis of spinach-leaf cell walls with Driselase (a fungal enzyme preparation) released two arabino-oligosaccharides and one galactobiose, each carrying a ferulic acid moiety. The oligosaccharides were characterized by NMR spectroscopy, methylation analysis, and FABMS. They were O-(2-O-trans-feruloyl-α-l-arabinofuranosyl)-(1 → 5)-l-arabinofuranose, O-(6-O-trans-feruloyl-β-d-galactopyranosyl)-(1 → 4)-d-galactopyranose, and O-α-l-arabinofuranosyl-(1 → 3)-O-(2-O-trans-feruloyl-α-l-arabinofuranosyl)-(1 → 5)-l-arabinofuranose.