RNA干扰
角膜新生血管
基因沉默
小干扰RNA
活性氧
脉络膜新生血管
脐静脉
新生血管
细胞生物学
材料科学
癌症研究
下调和上调
黄斑变性
细胞培养
生物
化学
血管生成
医学
核糖核酸
转染
生物化学
眼科
体外
遗传学
基因
作者
Anqi Liu,Chunjing Liang,Ji Liu,Yifei Huang,Ming Wang,Liqiang Wang
标识
DOI:10.1021/acsami.1c23412
摘要
Corneal neovascularization (CNV) is a common disease that affects the vision ability of more than 1 million people annually. Small interfering RNA (siRNA) delivery nanoparticle platforms are a promising therapeutic modality for CNV treatment. However, the efficient delivery of siRNA into cells and the effective release of siRNA from delivery vehicles in a particular cell type challenge effective RNAi clinical application for CNV suppression. This study reports the design of a novel reactive oxygen species (ROS)-responsive lipid nanoparticle for siRNA delivery into corneal lesions for enhanced RNAi as a potential CNV treatment. We demonstrated that lipid nanoparticles could efficiently deliver siRNA into human umbilical vein endothelial cells and release siRNA for enhanced gene silencing by using the upregulated ROS of CNV to promote lipid nanoparticle degradation. Moreover, the subconjunctival injection of siRNA nanocomplexes into corneal lesions effectively knocked down vascular endothelial growth factor expression and suppressed CNV formation in an alkali burn model. Thus, we believe that the strategy of using ROS-responsive lipid nanoparticles for enhanced RNAi in CNV could be further extended to a promising clinical therapeutic approach to attenuate CNV formation.
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