间质细胞
可视化
病理
骨髓
舱室(船)
免疫染色
间充质干细胞
生物
解剖
计算机科学
医学
人工智能
免疫组织化学
地质学
海洋学
作者
Nicolas Peredo,Roger Valle-Tenney,Seppe Melis,Marion Mesnieres,Elena Nefyodova,Christa Maes
出处
期刊:STAR protocols
[Elsevier]
日期:2022-03-01
卷期号:3 (1): 101222-101222
被引量:1
标识
DOI:10.1016/j.xpro.2022.101222
摘要
Advanced techniques combining tissue clearing, deep confocal imaging, and image quantification enable researchers to characterize complex tissue architectures and understand how trauma or disease impact on their respective components. Here, we detail methodologies to prepare, immunostain, and clear thick sections of mouse bones. We describe their use to visualize and quantitatively analyze marrow blood vessels and their spatial relationship with stromal skeletal stem/progenitor cells. These approaches can help unravel the stromal-vascular interplay in the bone marrow environment in health and disease. For complete details on the use and execution of this protocol, please refer to Mesnieres et al. (2021).
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