自体荧光
荧光团
荧光
双光子激发显微术
赫拉
化学
生物物理学
色氨酸
吸收(声学)
细胞
生物化学
材料科学
生物
光学
物理
复合材料
氨基酸
作者
Ting Wu,Jiuling Liao,Xiang Feng,Jia Yu,Yiping Huo,Yufeng Gao,Hui Li,Wei Zheng
出处
期刊:Optics Letters
[The Optical Society]
日期:2024-02-15
卷期号:49 (5): 1105-1105
摘要
Compared to intensity detection, fluorescence lifetime has the advantage of being unaffected by variations in excitation intensity, fluorophore concentration, or attenuation due to biological absorption and scattering. In this Letter, to the best of our knowledge, we present the use of the two-photon excitation autofluorescence lifetime imaging of tryptophan (TRP) to probe cell metabolism for the first time. Tests of pure chemical samples showed that the fluorescence lifetime of TRP was highly sensitive to changes in molecular conformation and the environment. In in vitro cell experiments, we successfully utilized the fluorescence lifetime of TRP to distinguish tumor cells from healthy cells, track the therapeutic effect of the tumor immunotherapy drug 1-MT for HeLa cells, and monitor cells in response to carbonyl cyanide 3-chlorophenylhydrazone (CCCP)-induced cell apoptosis. These results reveal that the two-photon excitation autofluorescence lifetime of TRP could be a sensitive natural probe of cell metabolism in living cells.
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