TGR5 in the Biliary Tree

G蛋白偶联胆汁酸受体 囊性纤维化跨膜传导调节器 氯离子通道 胆囊 利胆的 受体 胆道 胆管上皮细胞 胆汁酸 内科学 分子生物学 内分泌学 生物 化学 生物化学 囊性纤维化 医学
作者
Verena Keitel,Dieter Häussinger
出处
期刊:Digestive Diseases [S. Karger AG]
卷期号:29 (1): 45-47 被引量:46
标识
DOI:10.1159/000324127
摘要

TGR5 is a plasma membrane-bound, G-protein-coupled receptor for bile acids. TGR5 mRNA has been detected in a variety of tissues, including liver. The aim of the present study was to determine the localization and function of the receptor in biliary epithelial cells.Liver and gallbladder tissue from humans and rodents were analyzed for TGR5 expression and localization by real-time PCR, Western blot and immunofluorescence microscopy. Cholangiocytes and gallbladder epithelial cells were isolated from wild-type and TGR5 knockout mice. Cyclic AMP (cAMP) was measured using a radioimmunoassay and chloride concentrations were analyzed using the chloride-sensitive dye N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide (MQAE). Cell proliferation was determined by bromodeoxyuridine incorporation.TGR5 is localized in the apical membrane and the primary cilium of cholangiocytes and gallbladder epithelial cells. Activation of the receptor by bile acids led to a rise in intracellular cAMP concentrations and a decrease in intracellular chloride concentrations as measured by MQAE fluorescence, indicating increased chloride secretion. This effect could be abolished in the presence of an inhibitor of the cAMP-dependent chloride channel cystic fibrosis transmembrane conductance regulator. Furthermore, activation of TGR5 by bile acids induced cholangiocyte proliferation, which was not observed in cells derived from TGR5 knockout mice.In biliary epithelial cells, TGR5 acts as a bile acid sensor coupling biliary bile acid concentrations to bile formation. Furthermore, the receptor may play a role in bile acid-dependent cholangiocyte proliferation and may protect biliary epithelial cells from bile acid-induced cell death.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
今后应助Sssmmmyy采纳,获得10
刚刚
辣辣完成签到 ,获得积分10
4秒前
hxd完成签到,获得积分20
4秒前
奕初阳发布了新的文献求助10
6秒前
7秒前
7秒前
斯文败类应助顺利研兔子采纳,获得10
8秒前
刘广池完成签到,获得积分10
8秒前
红枫没有微雨怜完成签到 ,获得积分10
8秒前
辛勤若蕊完成签到,获得积分20
9秒前
希望天下0贩的0应助燕子采纳,获得10
10秒前
呆啊完成签到,获得积分10
11秒前
yyt发布了新的文献求助10
11秒前
siri完成签到,获得积分10
11秒前
Bonnie发布了新的文献求助50
11秒前
12秒前
追逐123完成签到 ,获得积分10
12秒前
rusellw完成签到,获得积分20
12秒前
刘广池发布了新的文献求助10
12秒前
13秒前
Jasper应助tutu采纳,获得30
13秒前
Lucas应助tutu采纳,获得10
13秒前
科研通AI2S应助tutu采纳,获得10
13秒前
在水一方应助tutu采纳,获得10
13秒前
8R60d8应助tutu采纳,获得10
13秒前
可爱的函函应助tutu采纳,获得10
14秒前
无花果应助tutu采纳,获得10
14秒前
传奇3应助tutu采纳,获得10
14秒前
yn完成签到 ,获得积分10
14秒前
汉堡包应助kk采纳,获得10
14秒前
李恩乐发布了新的文献求助10
15秒前
研友_VZG7GZ应助qiuling采纳,获得200
16秒前
X先生完成签到,获得积分10
17秒前
orixero应助大气摩托采纳,获得10
17秒前
七7发布了新的文献求助10
17秒前
myelin完成签到,获得积分10
18秒前
胖Q完成签到 ,获得积分10
19秒前
zho发布了新的文献求助10
20秒前
20秒前
俞安珊发布了新的文献求助10
20秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Very-high-order BVD Schemes Using β-variable THINC Method 830
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3248438
求助须知:如何正确求助?哪些是违规求助? 2891833
关于积分的说明 8268874
捐赠科研通 2559834
什么是DOI,文献DOI怎么找? 1388717
科研通“疑难数据库(出版商)”最低求助积分说明 650798
邀请新用户注册赠送积分活动 627775