化学
生物催化
戊二醛
产量(工程)
鸟苷
核苷
酶
有机化学
核化学
立体化学
生物化学
催化作用
反应机理
冶金
材料科学
作者
V. N. Barai,А. И. Зинченко,L. A. Eroshevskaya,Еlena N. Kalinichenko,Tamara I. Kulak,Igor A. Mikhailopulo
标识
DOI:10.1002/1522-2675(200207)85:7<1901::aid-hlca1901>3.0.co;2-c
摘要
The E. coli BMT-4D/1A cells have been selected according to Munch-Petersen et al. They carry two regulatory mutations (cytR and deoR) and are able to synthesize constitutively nucleoside-catabolizing enzymes, e.g., cells that possess high UPase and PNPase activities. The cells have been cross-linked by glutaraldehyde to afford a biocatalyst that retained high UPase and PNPase activities and was comfortable for repeated use. An incubation of 2′-deoxyguanosine (1) and 2-chloroadenine (2) (molar ratio 3 : 1) in K-phosphate buffer (10 mM; pH 7.0) in the presence of the biocatalyst at 65° for 7 h resulted in quantitative transformation of 2 into 2-chloro-2′-deoxyadenosine (4; cladribine) that was isolated in 81% yield (Scheme 1). Similarly, the reaction of guanosine (5) and 1,2,4-triazole-3-carboxamide (6) (molar ratio 1 : 1) in K-phosphate buffer (10 mM; pH 7.0) in the presence of the biocatalyst at 60° for 30 h led to the formation of 1-(β-D-ribofuranosyl)-1,2,4-triazole-3-carboxamide (8; ribavirin) in 90–92% yield (67–70% isolated yield) (Scheme 2).
科研通智能强力驱动
Strongly Powered by AbleSci AI