安普克
磷酸化
槲皮素
化学
下调和上调
未折叠蛋白反应
p38丝裂原活化蛋白激酶
细胞生物学
认知功能衰退
内质网
药理学
内科学
蛋白激酶A
生物化学
医学
生物
痴呆
抗氧化剂
基因
疾病
作者
Junjun Chen,Xueyang Deng,Ning Liu,Min Li,Baolin Liu,Qiang Fu,Rong Qu,Shiping Ma
标识
DOI:10.1016/j.jff.2016.01.036
摘要
Quercetin, a natural flavonoid abundantly found in onions, apples, tea, berries, cauliflower, and red wine, has been demonstrated to exert beneficial effect on Alzheimer's disease. We aimed to investigate its AMPK activity on hyperphosphorylation of tau and explore the underlying mechanism associated with Endoplasmic reticulum (ER) stress. Quercetin and quercetin-3-O-glucuronide suppressed ER stress with decreased phosphorylation of IRE1α and PERK, thereby inhibited TXNIP and NLRP3 inflammasome activation, ultimately attenuated tau phosphorylation in okadaic acid (OA) induced SH-SY5Y cells. However, the effect on tau phosphorylation was blocked by downregulation of AMPKα1/2 with SiRNA transfection. Moreover, administration of quercetin enhanced AMPK activity, inhibited IRE1α and PERK phosphorylation, NLRP3 expression and tau phosphorylation and improved cognitive disorder in mice exposed to high fat diets. AMPK may be a key player that links ER stress and tau phosphorylation and mediates quercetin's effect on cognitive disorder. Thus, quercetin and its AMPK activity may provide a potential therapeutic strategy for treatment of AD patients.
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