发酵
代谢工程
产量(工程)
化学
维生素
食品科学
工程类
生物化学
酶
冶金
材料科学
作者
Ziling Ye,Bin Shi,Yanglei Huang,Tian Ma,Zilei Xiang,Ben Hu,Zhaolin Kuang,Man Huang,Xiaoying Lin,Tian Zhu,Zixin Deng,Kun Shen,Tiangang Liu
出处
期刊:The Innovation
[Elsevier]
日期:2022-05-01
卷期号:3 (3): 100228-100228
被引量:29
标识
DOI:10.1016/j.xinn.2022.100228
摘要
Vitamin E is one of the most widely used vitamins. In the classical commercial synthesis of vitamin E (α-tocopherol), the chemical synthesis of isophytol is the key technical barrier. Here, we establish a new process for isophytol synthesis from microbial fermented farnesene. To achieve an efficient pathway for farnesene production, Saccharomyces cerevisiae was selected as the host strain. First, β-farnesene synthase genes from different sources were screened, and through protein engineering and system metabolic engineering, a high production of β-farnesene in S. cerevisiae was achieved (55.4 g/L). This farnesene can be chemically converted into isophytol in three steps with approximately 92% yield, which is economically equal to that from the best total chemical synthesis. Furthermore, we co-produced lycopene and farnesene to reduce the cost of farnesene. A factory based on this new process was successfully operated in Hubei Province, China, in 2017, with an annual output of 30,000 tons of vitamin E. This new process has completely changed the vitamin E market due to its low cost and safety.
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