Rational design of a ratiometric fluorescent probe for selective visualizing peroxynitrite in mitochondria and evaluation of organ injury

过氧亚硝酸盐 荧光 线粒体 化学 荧光团 生物化学 细胞生物学 生物物理学 生物 超氧化物 物理 量子力学
作者
Lingyan Liu,Ruohan Li,Feiyang Liu,Peng Wei,Tao Yi
出处
期刊:Sensors and Actuators B-chemical [Elsevier]
卷期号:419: 136421-136421
标识
DOI:10.1016/j.snb.2024.136421
摘要

Abnormal peroxynitrite (ONOO−) expression is closely associated with the development of various pathological processes. In this study, we devised a series of fluorescent probes, namely DHU-DCM-1, DHU-DCM-2, and DHU-DCM-3, tailored specifically for monitoring ONOO− through rationally molecular design. By modulating the electron-donating site within the fluorophore structure, the optimal probe DHU-DCM-3 exhibited excellent ratiometric detecting performance. DHU-DCM-3 displayed high selectivity and sensitivity towards ONOO−, effectively distinguishing it from other reactive oxygen species. Notably, DHU-DCM-3 was capable of labeling mitochondrial ONOO−, producing an enhanced fluorescence ratio (F656/F550), thereby allowing for the visualization of ONOO− fluctuations in both exogenous and endogenous contexts within living cells. The utility of DHU-DCM-3 was further demonstrated in various applications. For instance, through detecting elevated levels of ONOO−, senile C. elegans was successfully identified by utilizing DHU-DCM-3. Moreover, relying on its ratiometric fluorescence, DHU-DCM-3 was proved to be valuable in accurately diagnosing liver injury in mice with varying degrees of severity. Overall, we have constructed a ratiometric fluorescent probe for detecting and imaging ONOO− in both subcellular organelle and in vivo, highlighting its potential significance in various biomedical applications.
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