尿酸
类有机物
高尿酸血症
嘌呤
别嘌呤醇
体内
体外
痛风
生物化学
药理学
化学
细胞培养
生物
医学
细胞生物学
内科学
生物技术
酶
遗传学
作者
Chuanli Hou,Wanqian Sha,Zhenzhen Xu,Yang Hu,William Kwame Amakye,Maojin Yao,Jiaoyan Ren
出处
期刊:Food Chemistry
[Elsevier BV]
日期:2021-11-19
卷期号:374: 131634-131634
被引量:7
标识
DOI:10.1016/j.foodchem.2021.131634
摘要
Hyperuricemia (HUA) is a metabolic disease caused by disorders of purine metabolism, the prevalence of which has increased worldwide. At present, most drugs aimed at lowering uric acid have toxic side effects, and in vitro screening of uric acid-lowering active substances are inefficient. Here, a long-term 3D human liver organoid culture system with high uric acid for screening and evaluating the efficacy of uric acid-lowering functional compounds. This liver organoids can be established from single hepatocytes and grown for multiple months, while retaining key morphological and functional features. Furthermore, establishment of HUA organoids model was verified by antihyperuricemic drugs allopurinol, as well as reported bioactive peptides, which significantly reduced uric acid production in the liver organoids (p < 0.05). The results demonstrated that it has the potential to be used as a rapid and valid in vitro model to screen antihyperuricemic compounds that mimics in vivo cell growth patterns.
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