Sperm characterization and cryopreservation of the endangered freshwater fish Chirostoma estor (Atheriniformes)

低温保护剂 低温保存 精子 扩展器 人类受精 男科 精子活力 生物 运动性 化学 解剖 植物 胚胎 细胞生物学 医学 有机化学 聚氨酯
作者
Naiara Cristina Motta,Gilmara Junqueira Machado,Isabela Simas Ferreira,Jesús López García,Carlos Cristian Martínez Chavez,Carlos A. Martínez-Palacios,Luis David Solis Murgas
出处
期刊:Cryobiology [Elsevier]
卷期号:103: 81-86 被引量:3
标识
DOI:10.1016/j.cryobiol.2021.09.004
摘要

The knowledge of the physiology of sperm of an endangered species allows the implantation of reproductive biotechnologies that aim at conservation. The aim of this study was to characterize fresh sperm and evaluate different cryopreservation solutions for sperm in Chirostoma estor. The characterization of Chirostoma estor fresh sperm (n = 22 males) was performed through analyzes of sperm concentration, membrane integrity, sperm morphology, motility rate, motility quality score, and motility duration. For cryopreservation (n = 42 males), 3 extenders (BTS™, MIII™, or Androstar Plus™) in combination with 2 permeable cryoprotectants (dimethyl sulfoxide (DMSO) or methyl glycol (Methyl)) were used. Analyzes of post-thaw sperm were performed as described for fresh sperm and additionally the fertilization rate analysis was performed. Fresh sperm presented a sperm concentration of 29.2 × 109 spermatozoa/mL, membrane integrity of 82.4%, and morphologically normal cells of 53%. After glucose activation (150 mM) a motility rate of 87.5%, sperm quality score of 5.0, and a duration of motility of 285 s were observed. For post-thaw sperm, MIII + Methyl and Androstar + Methyl solutions resulted in the highest motility rates of 40-48%. No differences were observed for motility duration, membrane integrity, and sperm morphology. Samples cryopreserved in Methyl (12-20%) showed a higher fertilization rate than DMSO, independently of the extender. In conclusion, the fresh sperm collected artificially from Chirostoma estor presents a compatible quality to carry out fertilization and can be cryopreserved in the commercial extenders MIII™ and Androstar Plus™ together with the cryoprotectant Methyl glycol.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
刚刚
gumausi发布了新的文献求助10
刚刚
1秒前
2秒前
朴素太阳完成签到,获得积分20
2秒前
3秒前
luo发布了新的文献求助10
4秒前
5秒前
lalala发布了新的文献求助10
5秒前
6秒前
小小王完成签到 ,获得积分10
6秒前
傲娇的擎完成签到,获得积分10
6秒前
CodeCraft应助忧虑的绮梅采纳,获得10
7秒前
优秀的素完成签到,获得积分10
7秒前
唐展通完成签到,获得积分10
7秒前
Singularity发布了新的文献求助10
8秒前
Zqq发布了新的文献求助10
8秒前
CHL完成签到,获得积分10
8秒前
舒适的涑完成签到 ,获得积分10
8秒前
10秒前
胖打小张完成签到,获得积分10
10秒前
wanna完成签到,获得积分10
11秒前
11秒前
汉堡包应助路143250采纳,获得10
11秒前
11秒前
xjcy应助漂亮的笑柳采纳,获得10
11秒前
12秒前
火山排骨完成签到,获得积分10
12秒前
科研通AI2S应助舒服的听荷采纳,获得10
12秒前
12秒前
13秒前
林耀辉完成签到,获得积分20
13秒前
帆布鞋发布了新的文献求助10
14秒前
16秒前
16秒前
哦豁完成签到,获得积分20
17秒前
好酒不溅完成签到 ,获得积分10
17秒前
斯文败类应助008采纳,获得10
17秒前
17秒前
高分求助中
歯科矯正学 第7版(或第5版) 1004
SIS-ISO/IEC TS 27100:2024 Information technology — Cybersecurity — Overview and concepts (ISO/IEC TS 27100:2020, IDT)(Swedish Standard) 1000
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Semiconductor Process Reliability in Practice 720
GROUP-THEORY AND POLARIZATION ALGEBRA 500
Mesopotamian divination texts : conversing with the gods : sources from the first millennium BCE 500
Days of Transition. The Parsi Death Rituals(2011) 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3232862
求助须知:如何正确求助?哪些是违规求助? 2879539
关于积分的说明 8211826
捐赠科研通 2547041
什么是DOI,文献DOI怎么找? 1376491
科研通“疑难数据库(出版商)”最低求助积分说明 647648
邀请新用户注册赠送积分活动 623030