同色链霉菌
生物
聚酮
聚酮合酶
异源表达
链霉菌
放线菌素
基因
ATP合酶
链霉菌科
异源的
细菌
生物化学
放线菌
微生物学
遗传学
生物合成
重组DNA
作者
D J Bedford,Eckhart Schweizer,David A. Hopwood,Chaitan Khosla
标识
DOI:10.1128/jb.177.15.4544-4548.1995
摘要
The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that it is possible to harness the molecular diversity of eukaryotic polyketide pathways by heterologous expression of biosynthetic genes in an easily manipulated model bacterial host in which prokaryotic aromatic and modular polyketide synthase genes are already expressed and recombined.
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