间充质干细胞
癌症研究
体内
生物
淋巴瘤
细胞周期
细胞生长
流式细胞术
干细胞
细胞凋亡
病理
免疫学
医学
细胞生物学
生物技术
生物化学
遗传学
作者
Ji Yun Ahn,Ji-Sang Chae,Ye-Rin Coh,Woo‐Sung Jung,Hae‐Young Lee,Ilhoon Shin,Sa‐Ouk Kang,Hwa‐Young Youn
出处
期刊:PubMed
日期:2014-09-01
卷期号:34 (9): 4839-47
被引量:15
摘要
Human mesenchymal stem cells (hMSCs) are thought to be one of the most reliable stem cell sources for a variety of cell therapies. This study investigated the anti-tumor effect of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) on EL4 murine T-cell lymphoma in vitro and in vivo.The growth-inhibitory effect of hAT-MSCs on EL4 tumor cells was evaluated using a WST-1 cell proliferation assay. Cell-cycle arrest and apoptosis were investigated by flow cytometry and western blot. To evaluate an anti-tumor effect of hAT-MSCs on T-cell lymphoma in vivo, CM-DiI-labeled hAT-MSCs were circumtumorally injected in tumor-bearing nude mice, and tumor size was measured.hAT-MSCs inhibited T-cell lymphoma growth by altering cell-cycle progression and inducing apoptosis in vitro. hAT-MSCs inhibited tumor growth in tumor-bearing nude mice and prolonged survival time. Immunofluorescence analysis showed that hAT-MSCs migrated to tumor sites.hAT-MSCs suppress the growth of T-cell lymphoma, suggesting a therapeutic option for T-cell lymphoma.
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