Chemical constituents in stems of Zhuang Medicine Alsophila spinulosa

Objective To study the chemical constituents in the stems of Zhuang Medicine Alsophila spinulosa.Methods The chemical constituents were isolated and purified by silica gel,polyamide,and Sephadex LH-20 column chromatography.Their structures were elucidated by physicochemical properties and spectral analyses.Results Sixteen compounds were isolated from the 50% and 95% ethanol extracts and identified as 30-O-β-D-xylopyranosyl-dryocrassol(1),pimaric acid(2),9α-hydroxy-1βmethoxycaryolanol(3),6β-hydroxy-24-ethyl-cholest-4-en-3-one(4),1-O-hexadecanolenin(5),clovandiol(6),decumbic acid(7),n-tetracosane(8),4-O-β-D-glucopyranosyl-p-coumaric acid(9),(E)-4-O-β-D-glucopyranosyl caffeic acid(10),cyathenosin A(11),protocatechualdehyde(12),protocatechuic acid(13),stigmastane-3,6-dione(14),β-sitosterol(15),and daucosterol(16).Conclusion Compound 1 is a new compound named alspineoside A,and compounds 2-11 are isolated from the stems of A.spinulosa for the first time.