Development of a new rhodamine-based FRET platform and its application as a Cu2+ probe

We have constructed a new rhodamine-based FRET platform, which was then used to develop a ratiometric fluorescent Cu2+ probe. The novel Cu2+ probe exhibits several favorable features including a large variation in the emission ratio, well-resolved emission peaks, high sensitivity, and high selectivity. Importantly, it is suitable for fluorescence imaging in living cells.