A label-free electrochemical aptasensor based on a gold nanoparticle/carbon nanotube/metal–organic framework nanohybrid for ultrasensitive detection of streptomycin in milk samples

In this work, a novel electrochemical aptasensor was designed for the sensitive and specific detection of STR in milk samples. First, a gold [email protected](diallyldimethylammonium chloride) nitrogen-doped carbon nanotube/polyethyleneimine-functionalized metal–organic framework (MOF) ([email protected]/PEI–MIL-101(Cr)) composite was synthesized and characterized by various technique. The [email protected]/PEI–MIL-101(Cr) composite was then modified on a bare glassy carbon electrode (GCE) surface, providing a favorable platform ([email protected]/PEI–MIL-101(Cr)/GCE) for aptamer immobilization and current signal amplification. The STR aptamer was grafted to the [email protected]/PEI–MIL-101(Cr)/GCE through the formation of Au–S bonds and π–π stacking interactions. The immobilized STR aptamer binds specifically STR, resulting in an obvious decrease in the current signal. Under the optimal experimental conditions, the linear range of the electrochemical aptasensor for STR detection was 0.01–250 nM which the detection limit (LOD) was calculate as 2.31 nM. This strategy is expected to be a novel platform for the rapid and sensitive detection of STR.