The flower of Abelmoschus manihot (L.) Medik exerts antioxidant effects by regulating the Nrf2 signaling pathway in scald injury

Abstract Scald is a common skin injury in daily life. It is well known that skin burns are associated with inflammation and oxidative stress. In our previous study, we found that Abelmoschus manihot (L.) medik had excellent therapeutic effects on scald‐induced inflammation, but its effect on scald‐induced oxidative stress was not reported. In this study, a deep second‐degree scald model in mice was established, and the wound healing rate, healing time, malondialdehyde (MDA) and total superoxide dismutase (T‐SOD) levels, and nuclear factor erythroid 2‐related Factor 2 (Nrf2) expression in wound tissue were measured to evaluate the scald wound healing performance of e xtraction from A. manihot (L.) medik ( EAM ). Scalding activity in mice was examined in vivo by hot water‐induced finger swelling. The treatment scald activities were also examined in vivo by subjecting mice to thermal water‐induced digit swelling. Additionally, the antioxidant effect of EAM on fibroblasts was also used to determine the mechanism in vitro. The results showed that EAM not only decreased the wound healing time but also effectively regulated the levels of oxidising, MDA and T‐SOD in wound tissue. Concurrently, EAM suppressed digit swelling and hyperalgesia. Furthermore, EAM had a significant protective effect on NIH‐3T3 cells after H 2 O 2 injury by regulating the Nrf2 signalling pathway against oxidative injury. Therefore, EAM is a promising drug for the treatment of scald‐induced inflammation.