葡萄
体内
植物化学
脂质体
伤口愈合
生物信息学
化学
体外
传统医学
抗菌活性
药理学
植物
生物
医学
生物化学
细菌
生物技术
遗传学
基因
免疫学
作者
Abeer H. Elmaidomy,Soad A. Mohamad,Mahmoud Abdelnaser,Ramadan Yahia,Fatma Alzahraa Mokhtar,Faisal Alsenani,Moutaz Y. Badr,Safa Almaghrabi,Faisal H. Altemani,Mubarak A. Alzubaidi,Entesar Ali Saber,Mahmoud A. Elrehany,Usama Ramadan Abdelmohsen,Ahmed M. Sayed
出处
期刊:Food & Function
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:14 (15): 7156-7175
被引量:13
摘要
Vitis vinifera Egyptian edible leaf extract loaded on a soybean lecithin, cholesterol, and Carbopol gel preparation (VVL-liposomal gel) was prepared to maximize the in vivo wound healing and anti-MRSA activities for the crude extract, using an excision wound model and focusing on TLR-2, MCP-1, CXCL-1, CXCL-2, IL-6 and IL-1β, and MRSA (wound infection model, and peritonitis infection model). VVL-liposomal gel was stable with significant drug entrapment efficiency reaching 88% ± 3, zeta potential value ranging from -50 to -63, and a size range of 50-200 μm nm in diameter. The in vivo evaluation proved the ability of VVL-liposomal gel to gradually release the drugs in a sustained manner with greater complete wound healing effect and tissue repair after 7 days of administration, with a significant decrease in bacterial count compared with the crude extract. Phytochemical investigation of the crude extract of the leaves yielded fourteen compounds: two new stilbenes (1, 2), along with twelve known ones (3-14). Furthermore, a computational study was conducted to identify the genes and possible pathways responsible for the anti-MRSA activity of the isolated compounds, and inverse docking was used to identify the most likely molecular targets that could mediate the extract's antibacterial activity. Gyr-B was discovered to be the best target for compounds 1 and 2. Hence, VVL-liposomal gel can be used as a novel anti-dermatophytic agent with potent wound healing and anti-MRSA capacity, paving the way for future clinical research.
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