已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes

喷射 传质 传质系数 叶轮 悬挂(拓扑) 体积热力学 机械 材料科学 体积流量 流量(数学) 流体力学 锥面 环境科学 化学 热力学 物理 复合材料 物理化学 数学 同伦 纯数学
作者
Likuan Zhu,Boyan Song,Zhenlong Wang,Dominique T. Monteil,Xiao Shen,David L. Hacker,María de Jesús,Florian Μ. Wurm
出处
期刊:Biotechnology Progress [Wiley]
卷期号:33 (1): 192-200 被引量:36
标识
DOI:10.1002/btpr.2375
摘要

Orbitally shaken cylindrical bioreactors [OrbShake bioreactors (OSRs)] without an impeller or sparger are increasingly being used for the suspension cultivation of mammalian cells. Among small volume OSRs, 50‐mL tubes with a ventilated cap (OSR50), originally derived from standard laboratory centrifuge tubes with a conical bottom, have found many applications including high‐throughput screening for the optimization of cell cultivation conditions. To better understand the fluid dynamics and gas transfer rates at the liquid surface in OSR50, we established a three‐dimensional simulation model of the unsteady liquid forms (waves) in this vessel. The studies verified that the operating conditions have a large effect on the interfacial surface. The volumetric mass transfer coefficient ( k L a) was determined experimentally and from simulations under various working conditions. We also determined the liquid‐phase mass transfer coefficient ( k L ) and the specific interfacial area ( a ) under different conditions to demonstrate that the value of a affected the gas transfer rate more than did the value of k L . High oxygen transfer rates, sufficient for supporting the high‐density culture of mammalian cells, were found. Finally, the average axial velocity of the liquid was identified to be an important parameter for maintaining cells in suspension. Overall these studies provide valuable insights into the preferable operating conditions for the OSR50, such as those needed for cell cultures requiring high oxygen levels. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:192–200, 2017

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Murray应助郑鹏飞采纳,获得10
2秒前
2秒前
花花完成签到,获得积分10
3秒前
4秒前
共享精神应助hh采纳,获得10
5秒前
Jasper应助小苏打采纳,获得10
5秒前
9秒前
dota1dota26完成签到,获得积分10
9秒前
所所应助科研通管家采纳,获得10
11秒前
无花果应助科研通管家采纳,获得10
11秒前
Orange应助科研通管家采纳,获得30
11秒前
英姑应助科研通管家采纳,获得10
11秒前
星辰大海应助科研通管家采纳,获得10
11秒前
NexusExplorer应助科研通管家采纳,获得10
11秒前
酷波er应助科研通管家采纳,获得10
11秒前
慕青应助嘟嘟采纳,获得10
12秒前
Jasper应助marstar采纳,获得10
12秒前
16秒前
领导范儿应助潇洒忘幽采纳,获得10
19秒前
水野发布了新的文献求助10
20秒前
科研通AI2S应助兴奋的故事采纳,获得10
26秒前
英俊的铭应助Christoph_Lee采纳,获得10
26秒前
27秒前
27秒前
30秒前
31秒前
32秒前
大胆小丸子完成签到,获得积分10
33秒前
夜白应助直率的冷梅采纳,获得20
33秒前
Greenhand完成签到,获得积分10
34秒前
慕青应助痴情的绮菱采纳,获得10
38秒前
samar发布了新的文献求助10
39秒前
haveatry发布了新的文献求助30
39秒前
淡定的萝莉完成签到 ,获得积分10
41秒前
云中漫步完成签到,获得积分10
42秒前
44秒前
彭于晏应助赖皮蛇采纳,获得10
46秒前
46秒前
⊙▽⊙发布了新的文献求助10
49秒前
木子倪完成签到,获得积分10
51秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Very-high-order BVD Schemes Using β-variable THINC Method 830
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3248529
求助须知:如何正确求助?哪些是违规求助? 2891960
关于积分的说明 8269265
捐赠科研通 2559983
什么是DOI,文献DOI怎么找? 1388824
科研通“疑难数据库(出版商)”最低求助积分说明 650913
邀请新用户注册赠送积分活动 627798