泛酸
核黄素
吡哆醇
化学
硫胺素
食品科学
玉米浆
烟酸
色谱法
维生素
原材料
B族维生素
作文(语言)
萃取(化学)
生物化学
生物
有机化学
发酵
哲学
内分泌学
语言学
作者
Alexandra Hofer,Christoph Herwig
摘要
Abstract BACKGROUND Although raw materials such as corn steep liquor (CSL) are commonly used as supplements in media for biotechnological production of valuable products such as antibiotics, however, determination of the composition of CSL is still a scarcely explored field of research. Especially for some B‐vitamins, which are badly soluble and compose just a small fraction in CSL, no adequate analytical method has been published yet. RESULTS In this study we adopted a RP‐HPLC‐UV method for the simultaneous determination of nine water‐soluble vitamins (biotin, cyanocobalamin, folic acid, niacin amide, nicotinic acid, pantothenic acid, pyridoxine, riboflavin, thiamine) and evaluated six different methods for the extraction of vitamins from CSL (two liquid–liquid extractions and four enzymatic extractions). The proposed method showed relative errors of about 2%; LOD and LOQ were ranged between 0.07 and 0.875 µg mL −1 and 0.2 and 2.5 µg mL −1 , respectively, deviating just for the low absorbing pantothenic acid. The method was successfully applied to eight CSL samples which differed in manufacturer and corn quality (dried or wet corn). Seven out of nine vitamins could be identified in CSL and correlations between some vitamins and manufacturer (i.e. nicotinic acid, pantothenic acid and biotin) and quality (i.e. thiamine, pyridoxine and cyanocobalamin), respectively, could be observed. CONCLUSION The variation in vitamin composition found in the different CSL samples points out the importance of an incoming inspection of CSL to reduce variations in bioprocesses which are caused by raw material variability. This contribution provides a valuable quantitative tool for this task. © 2017 Society of Chemical Industry
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